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tmb developing reagent  (Biosynth Carbosynth)


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    Structured Review

    Biosynth Carbosynth tmb developing reagent
    Tmb Developing Reagent, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmb+developing+reagent/Tetramethylbenzidine/pmc11175361-57-13-16
    Average 91 stars, based on 2 article reviews
    tmb developing reagent - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    other:

    Article Title: Targeting of TAM Receptors Ameliorates Fibrotic Mechanisms in Idiopathic Pulmonary Fibrosis
    Article Snippet: Cells where then washed and the reaction was developed using 100 μl of TMB developing reagent (Fitzgerald Industries International) until sufficient color was observed.

    Article Title: Translational Studies Reveal the Divergent Effects of Simtuzumab Targeting LOXL2 in Idiopathic Pulmonary Fibrosis
    Article Snippet: Cells where then washed and the reaction was developed using 100 μL of TMB developing reagent (Fitzgerald Industries International, Acton, MA, USA) until sufficient color was observed.

    Article Title: Translational Studies Reveal the Divergent Effects of Simtuzumab Targeting LOXL2 in Idiopathic Pulmonary Fibrosis.
    Article Snippet: Cells where then washed and the reaction was developed using 100 μL of TMB developing reagent (Fitzgerald Industries International, Acton, MA, USA) until sufficient color was observed.



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    A Representative scheme of our homemade ELISA test, i.e. ELEXO, including EV-coated microtiter plate, primary antibody, HRP-conjugated secondary antibody and colorimetric reaction <t>with</t> <t>TMB</t> substrate. B H1975 and H1299 EVs analyzed by ELEXO assay. IgG isotype and Phosphate-Buffered Saline (PBS) were used as internal controls. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm) (* p = 0.02). C , D Representative images of SEM ( C ) and TEM ( D ) analysis of EVs isolated from the sera of prostate cancer patients. Images are representative of pivotal and training cohort PCas. E Box plot representative of serum EV size distribution evaluated by SEM analysis. F , G ELEXO measurement of EpCAM ( F ) and PD-L1 ( G ) antigens in EVs isolated from colon and lung cancer patients, respectively. CD81 has been used as endogenous EV control. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm). Internal reference controls were reported as IgG, CD-81, EpCAM, or PD-L1 antibody in PBS condition. H Absolute RPPA quantification of EGFR_pY1068 synthetic peptide in dilution curve along with isolated EVs from sera of colon and lung cancer patients. The expression levels in EVs fall below the lowest EGFR_pY1068 peptide dilution point and their scale is magnified in the left panel of the plot. The reference curve in the lower panel was obtained as in Fig. to predict absolute EGFR_pY1068 RPPA levels in EV samples.
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    Image Search Results


    A Representative scheme of our homemade ELISA test, i.e. ELEXO, including EV-coated microtiter plate, primary antibody, HRP-conjugated secondary antibody and colorimetric reaction with TMB substrate. B H1975 and H1299 EVs analyzed by ELEXO assay. IgG isotype and Phosphate-Buffered Saline (PBS) were used as internal controls. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm) (* p = 0.02). C , D Representative images of SEM ( C ) and TEM ( D ) analysis of EVs isolated from the sera of prostate cancer patients. Images are representative of pivotal and training cohort PCas. E Box plot representative of serum EV size distribution evaluated by SEM analysis. F , G ELEXO measurement of EpCAM ( F ) and PD-L1 ( G ) antigens in EVs isolated from colon and lung cancer patients, respectively. CD81 has been used as endogenous EV control. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm). Internal reference controls were reported as IgG, CD-81, EpCAM, or PD-L1 antibody in PBS condition. H Absolute RPPA quantification of EGFR_pY1068 synthetic peptide in dilution curve along with isolated EVs from sera of colon and lung cancer patients. The expression levels in EVs fall below the lowest EGFR_pY1068 peptide dilution point and their scale is magnified in the left panel of the plot. The reference curve in the lower panel was obtained as in Fig. to predict absolute EGFR_pY1068 RPPA levels in EV samples.

    Journal: Cell Death & Disease

    Article Title: Diagnostic and prognostic potential of the proteomic profiling of serum-derived extracellular vesicles in prostate cancer

    doi: 10.1038/s41419-021-03909-z

    Figure Lengend Snippet: A Representative scheme of our homemade ELISA test, i.e. ELEXO, including EV-coated microtiter plate, primary antibody, HRP-conjugated secondary antibody and colorimetric reaction with TMB substrate. B H1975 and H1299 EVs analyzed by ELEXO assay. IgG isotype and Phosphate-Buffered Saline (PBS) were used as internal controls. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm) (* p = 0.02). C , D Representative images of SEM ( C ) and TEM ( D ) analysis of EVs isolated from the sera of prostate cancer patients. Images are representative of pivotal and training cohort PCas. E Box plot representative of serum EV size distribution evaluated by SEM analysis. F , G ELEXO measurement of EpCAM ( F ) and PD-L1 ( G ) antigens in EVs isolated from colon and lung cancer patients, respectively. CD81 has been used as endogenous EV control. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm). Internal reference controls were reported as IgG, CD-81, EpCAM, or PD-L1 antibody in PBS condition. H Absolute RPPA quantification of EGFR_pY1068 synthetic peptide in dilution curve along with isolated EVs from sera of colon and lung cancer patients. The expression levels in EVs fall below the lowest EGFR_pY1068 peptide dilution point and their scale is magnified in the left panel of the plot. The reference curve in the lower panel was obtained as in Fig. to predict absolute EGFR_pY1068 RPPA levels in EV samples.

    Article Snippet: After washing, the color-developing reaction with TMB (Thermoscientific, Massachusetts, USA) was started and then stopped with H2SO4.

    Techniques: Enzyme-linked Immunosorbent Assay, Saline, Isolation, Control, Expressing